Abstract
A spatial-resolved electrochemiluminescence (ECL) aptasensing platform for sensitive protein detection was constructed in a double-check mode. CdS-C composites as cathodic ECL emitters and luminol-gold nanoparticles (L-Au NPs) as anodic ECL emitters were immobilized on working electrode 1 (WE1) and working electrode 2 (WE2) of the dual-disk glassy carbon electrodes (DDCE), respectively. The platform was designed by integrating silver-polyamidoamine NCs-labeled biotin-aptamer (Ag-PAMAM-bio-Apt) with the ECL emitters on the DDCE. The in-situ consumption of co-reactants in the ECL system by Ag-PAMAM NCs resulted in the great ECL quenching on both WE1 and WE2. The resonance energy transfer between L-Au NPs and Ag-PAMAM NCs made the ECL on WE2 further decrease. In the presence of carcinoembryonic antigen (CEA), portions of Ag-PAMAM-bio-Apt detached from the DDCE because of the competitive reaction of CEA and cDNA toward to Ag-PAMAM-bio-Apt, generating an enhancement of ECL intensity. This strategy achieves the detection of CEA in a double-check mode with detection limits of 0.39 pg mL−1 for the cathodic assay (WE1) and 0.20 pg mL−1 for the anodic assay (WE2), respectively. Furthermore, the proposed method had been successfully applied to determine CEA in human serum sample with recoveries of 80.0–110.0%, showing great potential in cancer diagnosis.
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