Abstract
A fluorometric method for determination of estriol in pregnancy plasma has been established. The procedure includes hydrolysis in a boiling water bath, use of unconjugated radioactive estriol to determine methodologic losses, solvent extractions, and the Ittrich-Kober reaction with the use of tetrachloroethane for extraction of the Kober chromogens. The method is sensitive, reproducible, and rapid. A number of drugs routinely used in obstetric care caused no interference. Serial determinations in 20 patients have been performed, in addition to over 300 isolated determinations. The values ranged from 10 to 58 μg per 100 ml. of plasma between the thirty-seventh and fortieth weeks of uncomplicated pregnancies. Fetal umbilical venous and arterial values were comparable but were severalfold higher than maternal levels. The method has been applied to amniotic fluid, and the results were contrasted with levels in plasma.
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