Abstract
If a staining-permanent slide is not needed, this simple and faster microtome technique is very useful for cross-sectioning gymnosperm needle leaves. The technique employs 2× polyvinyl alcohol-lactic acid-glycerol (PVLG) without the steps of fixation, dehydration, clearing, embedding, deparaffinisation, and hydration. Conveniently, this technique can also be applied to thick leaves and other organs of angiosperms. The procedure is very simple and is suggested for use in plant anatomy research.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
