Abstract
Abstract2,6‐bis((E)‐(2‐(benzo[d]thiazol‐2‐yl)hydrazono)methyl)‐4‐(4,5‐diphenyl‐1H‐imidazol‐2‐yl)phenol (PIS) conjugate has been designed as a selective fluorescent probe for zinc ion. The PIS exhibits selectivity towards Zn2+ among various ions in DMSO‐H2O (1:9) v/v 50 mM HEPES (4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid) at pH=7.4. Moreover, the detection of PIS towards Zn2+ ion could be realized in “naked eye” with a color conversion from yellow to greenish yellow. The linear relationship between fluorescence intensity and Zn2+ ion concentration indicates that PIS can be used for quantification of Zn2+ ion. The PIS probe can be used in broad pH ranging from 4 to 9. The limit of detection (LOD) was found to be 0.52 nM for Zn2+ ion. The binding ratio and binding constant of PIS‐Zn2+ complex were determined and calculated as 1:1 and 2.36×106 M−1 according to Job′s plot and Benesi–Hildebrand equation, respectively. Furthermore, the PIS‐Zn2+ complex subsequently acted as a secondary fluorescent turn‐off probe for pyrophosphate (PPi) ion recognition among the other anions. To assess the real‐world efficacy of the PIS and PIS‐Zn2+ complex, they were evaluated by test paper strips. Likewise, the probe PIS and PIS‐Zn2+ complex were used as in vitro cell imaging probe, and successfully demonstrated as feasible bio‐marker as well.
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