Abstract
Since HOCl plays an indispensable role in a variety of physiological and pathological processes, monitoring the level of hypochlorous acid (HOCl) in living cells is critical. Based on this, it is necessary to develop a convenient and simple technique to detect HOCl in living cells. In this contribution, a simple 2-hydroxy-6-morpholino-1H-benzo[de]isoquinoline-1,3(2 H)-dione (NDS) was developed as fluorescent probe for monitoring HOCl in live cells. Probe NDS exhibited preeminent specificity towards HOCl over other various bioactive substances. In addition, the probe could respond to HOCl in solutions within 16 s and the limit of detection was as low as 105.24 nM. NDS has good lysosomal targeting and has been successfully applied to the expression of exogenous and endogenous lysosomal HOCl in HeLa cells. This study provides a promising tool for studying physiological or pathological related processes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Photochemistry and Photobiology A: Chemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
