Abstract
IN 1951, we described a semimicro chromatographic method for the separation and determination of the C4–C10 volatile fatty acids1. In that publication a description was given of the preparation of a silica gel which proved to be highly suitable for this separation. In that method, which is based on the principle of Ramsey and Patterson2, the fatty acids up to C10 are separated on a column of silicic acid, using methanol as the immobile solvent, isooctane as the mobile solvent and bromcresol green as the indicator. Since this method is quick and simple and never fails to give excellent results, we have tried to work out an identical method for the determination of the higher saturated fatty acids C10–C20.
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