Abstract
Abstract Unexpected fluorescence signal changes observed by analysts are frequently due to lamp instability and interferences from other equipment near the fluorometer; The addition of a constant voltage transformer between the line and the power supply is an inexpensive and simple procedure to provide long term stability of the lamp, which leads to more reliable use of the fluorometer. Interference problems were significantly reduced by adding a filter system to the instrument. The problem of long term stability and interferences from nearby electronic instrumentation are frequently observed by individuals using spectrophotofluorometers. Unexpected fluorescence intensity differences for samples which are believed to contain similar quantities of an analyte are an outward manifestation of these problems, which may lead to the incorrect report of the concentration of the compound of interest. We have observed such difficulties in a study of the effect of solvent composition on the luminescence of tyrosine ...
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