Abstract
Mercury contamination is a major environmental concern. In this work, we used a cleavable phosphorothioate (PS) fluorescence probe quenched by UiO-66-NH2 to develop a "signal-on" fluorescent biosensor for Hg2+ detection. The probe was bound to UiO-66-NH2 through π-π stacking and hydrogen bonding, thereby extinguishing the fluorescence of the FAM-labelled probe. The PS site was cleaved in the presence of Hg2+, releasing the FAM group and significantly enhancing the fluorescence signal. The intensity of the fluorescence linearly rose as the Hg2+ concentration increased in the range of 1-100 nM (R2 = 0.994), and the limit of detection was 0.118 nM (S/N = 3). This biosensor demonstrated high selectivity for Hg2+ and was effectively applied to quantification of Hg2+ in various water samples with acceptable recovery rates. These results suggest that this practical, straightforward technology is a good option for monitoring mercury ions in the environment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
