Abstract
IntroductionAlmonds are an extremely rich source of unsaturated lipids and flavonoids and increased consumption may be recommended to achieve various health benefits. However, there are no known analytical methods to document compliance to chronic almond consumption.ObjectiveTo develop an analytical approach that identifies metabolic profiles associated with long‐term almond intake to ascertain compliance with prescribed consumption.MethodsA shotgun fingerprinting strategy was designed to isolate metabolic changes in erythrocytes after 12 weeks of almond consumption in a weight‐loss study. Representative samples (pools) of erythrocytes from individuals consuming almonds or no almonds were screened by flow injection mass spectrometry. Data based on scan modes for shotgun lipidomics, and m/z values compatible with almond flavonoid content were collected. Specific ion pairs and m/z values detected in pooled samples were combined into single ion monitoring and multiple reaction monitoring methods intended for individual sample screening. Values of ion intensities were normalized by total ion current to obtain relative ion intensities.ResultsOut of the 243 values of m/z monitored by both methods, 5 ratios and combinations of specific ions with receiver operating characteristic (ROC) curves AUC>0.89 had sensitivity of 74.2% and specificity of 90%. Eight out of the 31 participants (25.8%) in the almond group and 3 out of the 30 (10%) participants in the control group were misclassified by all 5 ratios.ConclusionRatios and combinations of specific ions mainly related to membrane lipids were discriminatory of almond consumption from the nut‐free diet. The relationship between these metabolites indicates almond‐induced changes in the metabolomics profile and the misclassifications observed as a result of ratio performance evaluation could be indications of possible non‐compliance as supported by analysis of dietary intake data.Support or Funding InformationAlmond Board of California
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
