Abstract
A sensitive method for the quantitative spectrophotometric determination of pyridine is described. The color reaction is based on the reaction of pyridine with cyanogen-bromide to form glutaconic aldehyde, which is subsequently coupled with anthranilic acid. The yellow polymethine dye formed shows an absorbance maxima at 470 nm, with a Sandell's sensitivity of 0.004 μg cm −2. The reaction is very sensitive and follows Beer's law in the range of 4 to 30 μg per 10 ml of the final solution of pyridine. The optimum reaction conditions such as pH, time, temperature, reagent concentrations, etc. for complete color reaction have been evaluated. The method has been successfully applied for the determination of pyridine in polluted water, aromatic hydrocarbons, and laboratory air.
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