Abstract
Millions of people died because of tuberculosis (TB) around the world every year. Due to the existing problems of detection, such as the accuracy, expenditure and speed of detection. It is necessary to develop a more effective, economical and practical detection method. In this study, a rapid, sensitive and highly selective electrochemical aptasensor is developed for the detection of MPT64 which is secreted by Mycobacterium tuberculosis (MTB) in early stage. The Prussian blue nanoparticles (PBNPs) was successfully synthesized and the surface charge was changed by using the Poly (diallyl dimethyl ammonium chloride) (PDDA). Au nanoparticles (AuNPs) were linked on the PBNPs by electrostatic adsorption, after that, the aptamer and horseradish peroxidase were casted on the Au@PBNPs worked as the signal aptamer. The constructed aptasensor has excellent performance with a wide liner range between 1 and 10,000 pg·mL−1, and the detection limit reaches 21 fg·mL−1. The aptasensor is highly selective which is hard to be infected by other potential substances. The results show the aptasensor is reliable for the detection of MPT64 in early phase.
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