Abstract
SUMMARY An algal assay capable of detecting the carrying capacity of nutrient-poor rivers was developed. In order to eliminate carry-over of nutrients, thus masking the true nutrient condition of the water tested, a new growth medium was developed. This maintenance medium contained less than 4 mg · 1−1 total inorganic salts. With an inoculum of 104 cells-ml−1 the test organism Selenastrum capricornutum Printz was grown in sterile-filtered, river water and transferred daily with a 1:1 dilution. The assay was carried out for 4 or 5 days, at which time cell number was calculated, and the doublings per day were determined. Comparisons are made with similar but less sensitive assays, on the basis of both daily and 5-day readings.
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