Abstract
Laser speckle imaging (LSI) is a well-known and useful approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues (such as brain vasculature, skin capillaries etc.). Despite an extensive use of LSI for brain imaging, the LSI technique has several critical limitations. One of them is associated with inability to resolve a functionality of vessels. This limitation also leads to the systematic error in the quantitative interpretation of values of speckle contrast obtained for different vessel types, such as sagittal sinus, arteries, and veins. Here, utilizing a combined use of LSI and fluorescent intravital microscopy (FIM), we present a simple and robust method to overcome the limitations mentioned above for the LSI approach. The proposed technique provides more relevant, abundant, and valuable information regarding perfusion rate ration between different types of vessels that makes this method highly useful for in vivo brain surgical operations.
Highlights
During the last decade, a highly detailed in vivo imaging of the brain vascular network became a hot topic for the biophotonics community
We present a further development of the laser speckle imaging (LSI)–fluorescent intravital microscopy (FIM) technique for the transcranial mouse brain visualization by using the correction of speckle contrast values of the brain vasculature functional identification
The LSI mode utilized a 3 mW laser diode module (LDM808/3LJ, Roithner Lasertechnik, Vienna, Austria), emitting light at 808 nm, which was further expanded by a ground glass diffuser (Thorlabs, Newton, NJ, USA) and illuminated the mouse head
Summary
Vyacheslav Kalchenko 1, *,† , Anton Sdobnov 2,† , Igor Meglinski 2,3,4,5,6 , Yuri Kuznetsov 1 , Guillaume Molodij 1 and Alon Harmelin 1. Optoelectronics and Measurement Techniques Laboratory, University of Oulu, 90570 Oulu, Finland. Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, 634050 Tomsk, Russia. Aston Institute of Materials Research, School of Engineering and Applied Science, Aston University, Birmingham B4 7ET, UK. These two authors contributed to this work
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