Abstract

Although there are many papers in the literature on materials and procedures for the embedding of tissue for transmission electron microscopy (TEM), most recent publications have emphasized techniques for specialized applications. Although these may in many cases also be suitable for routine applications in addition to the specialized applications for which they were developed, this may not be clear from the literature. This paper describes the formulation and suggested procedures for the use of an epoxy resin mixture which has been routinely used by novice and experienced workers with success for a wide variety of biological TEM investigations in a multi-user multi-disciplinary EM facility. Results are given of the use of this embedding medium in the investigation of a variety of tissue types.

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