Abstract
Reducing agent SO2 and oxidant H2O2 are two essential substances in cells, and the balance between them is closely related to the survival of cells. SO2 derivative HSO3− is often used as food additive. Therefore, simultaneous detection of SO2 and H2O2 is of great significance in biology and food safety. In this work, we successfully developed a mitochondria-targeted red fluorescent probe (HBTI), which has excellent selectivity, high sensitivity and large Stokes shift (202 nm). HBTI and HSO3−/SO32− undergo Michael addition on the unsaturated C=C bond, and the addition product (HBTI-HSO3−) can react with H2O2 to restore the conjugated structure. Fluorescence changes from red to non-emissive and then restores to red, and can be detected quickly and visually. In addition, HBTI has been successfully targeted mitochondria, and achieved dynamic reversible response to SO2/H2O2 in living cells, and has been successfully applied to detect SO2 in food samples.
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