Abstract
A new rate-zonal density gradient centrifugation procedure for sequential product recovery was used to analyze the sedimentation characteristics of subcellular particles of rat liver and tumor cells utilizing a B-XXIX edge-unloading zonal rotor. Particle sedimentation was measured over a range of forces necessary for sedimenting mitochondria, lysosomes, and peroxisomes. Even though the populations overlapped, a discrete sizing of the various particles was achieved. Ninety percent of the mitochondria had a s o 20 ω of 2.8–7.3×10 4 S. Lysosomes were distributed throughout the whole range of particle sizes separated. However, 80% of the lysosomes shown to bind the element gallium were found in a small-granule zone, the population of the particles having a s o 20 ω of 0.8–2.2×10 4 S.
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