Abstract
Denatured T4 DNA mixed with specific antibody forms a complex which is retained by a glass fiber filter. The retention of this complex is dependent on the size of the DNA: single-stranded fragments with a molecular weight greater than 10 6 are retained, whereas fragments with a molecular weight of less than 2 · 10 4 are not retained. The effects of pH, temperature, NaCl concentration, antiserum concentration, and DNA concentration on the formation and retention of antibody-DNA complexes have been studied. This technique can be used as an endonuclease assay and will detect 1 ng pancreatic deoxyribonuclease. It is 100 times more sensitive than an acid-solubility assay. This endonuclease assay was used to monitor the purification of Mustelis canis liver endonuclease.
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