Abstract
A simple, rapid and sensitive method was devised to detect specifically antioxidative substances on a thin-layer chromatography (TLC) plate. After the chromatography on a silica gel plate, the plate was sprayed with a pigment solution and irradiated with ultraviolet light. Only the spots of antioxidative ability were embossed as the background pigment was bleached. Among the pigments tested, paprika pigment (mainly capsanthin), capsanthin, and canthaxanthin were excellent, and p-carotene and astaxanthin were adequate for the spray reagents. Using the paprika spray reagent, antioxidants added to olive oil and tocopherols in a brown alga Ishige okamurae were clearly detected on the TLC plates. Generally, a few minutes of ultraviolet light irradiation with a commercially available illuminator (2000 pW) were sufficient, and less than one pg of antioxidative substances on the silica gel plate was detectable.
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Schedule a callMore From: Journal of the Faculty of Agriculture, Kyushu University
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