Abstract
Hydrogels are widely used as scaffolds in tissue engineering because they can provide excellent environments for bioactive components including growth factors and cells. We reported in this study on a physical hydrogel formed by a specific protein-peptide interaction, which could be used for the three dimensional (3D) cell culture of murine mesenchymal stem cells (mMSC). The mMSC kept dividing during the 7-day culture period and the metabolic-active cell number at day 7 was 359% more than that at day 1. This kind of physical hydrogel could be converted to a homogeneous solution by firstly adding an equal volume of culture medium and then pipeting for several times. Therefore, mMSC post culture could be easily separated from cell-gel constructs. We believed that the protein-based hydrogel system in this study could be developed into a promising scaffold for in vitro expansion of stem cells and cell therapy. This work would be in the general interests of researchers in the fields of biomaterials and supramolecular chemistry.
Highlights
The generation of genetically encoded protein-based hydrogels for an array of applications, such as tissue regeneration, 3D cell culture and drug delivey, has been much anticipated[1,2,3,4,5,6]. 3D cell culture is evolving rapidly
We opted to develop hydrogels based on ULD-TIP-1 and polymers and test whether they were suitable for 3D cell culture or not
The simple mixing strategy for hydrogelation was convenient and biocompatible to cells encapsulation, which could guarantee its future applications in 3D cell culture and controlled delivery of pharmaceutical agents
Summary
The generation of genetically encoded protein-based hydrogels for an array of applications, such as tissue regeneration, 3D cell culture and drug delivey, has been much anticipated[1,2,3,4,5,6]. 3D cell culture is evolving rapidly. The generation of genetically encoded protein-based hydrogels for an array of applications, such as tissue regeneration, 3D cell culture and drug delivey, has been much anticipated[1,2,3,4,5,6]. Hydrogels formed by a specific proteinpeptide interaction can respond to external stimuli such as ionic strength change[22] and calcium addition[4]. They hold big potential for controlled drug delivery, cells encapsulation, and cells delivery[20,21,25,26,27,28,29]
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