Abstract
To clarify the characteristic growth of melanocytes (MCs) and Keratinocytes (KCs) in vitro and discuss the mechanism of culturing autologous melanocytes in the treatment of vitiligo. Epidermis cells derived from normal skin tissues were isolated and cultured in vitro. Melanocytes in DOPA staining were observed. The expression level of markers in MCs was detected by qRT-PCR and the percentage of MCs and KCs were detected by flow cytometry. Cells derived from normal skin tissues mainly included KCs, MCs, and fibroblasts. There were significant differences between the percentage of KC, MC, fibroblasts (P < 0.05), and the expression of Microphthalmia-associated transcription factor (P < 0.05) and Tyrosinase-related protein-2 (P < 0.05) in the second, 10th, 20th, and 30th day. Significant differences were also found between the average numbers of MC stained by DOPA (P < 0.05) and the average percentage of MCs in the 10th, 20th, and 30th Day (P < 0.05). But there were no significant differences between the average percentage of KCs in the 10th, 20th, and 30th Day (P > 0.05) detected by flow cytometry. The number of MCs co-cultured with KCs in vitro reached the maximum in the 20th Day and this co-cultured model may contribute to the growth of MCs which could be used in the treatment of vitiligo.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
