Abstract
A platform compatible with microtiter plates to parallelize environmental treatments to test the complex impacts of multiple stressors, including parameters relevant to climate change and point source pollutants is developed. This platform leverages (1) the high rate of purely diffusive gas transport in aerogels to produce well‐defined centimeter‐scale gas concentration gradients, (2) spatial light control, and (3) established automated liquid handling. The parallel gaseous, aqueous, and light control provided by the platform is compatible with multiparameter experiments across the life sciences. The platform is applied to measure biological effects in over 700 treatments in a five‐parameter full factorial study with the microalgae Chlamydomonas reinhardtii. Further, the CO2 response of multicellular organisms, Lemna gibba and Artemia salina under surfactant and nanomaterial stress are tested with the platform.
Highlights
Introduction predict future atmosphericCO2 concentrations—as affected by primary productivity feedback.[15,16] At the organism level, Globally, communities are coping with warming in combination with increased CO2 levels, occurring concurrently with local environmental stressors from synthetic chemiresponses to elevated CO2 concentrations are multifaceted, especially when combined with other environmental parameters.[5,16] At the community level, this complexity is comcals.[1,2,3,4] The challenge of predicting biological responses pounded by trophic interactions.[17]
Communities are coping with warming in combination with increased CO2 levels, occurring concurrently with local environmental stressors from synthetic chemiresponses to elevated CO2 concentrations are multifaceted, especially when combined with other environmental parameters.[5,16]
In contrast to slower diffusion-based gradient generators, our numerical simulations show that the gradient in the aerogel device is not significantly perturbed (
Summary
A platform compatible with microtiter plates to parallelize environmental (e.g., temperature, light, gas, chemical, and a nanomaterial pollutants) are chaltreatments to test the complex impacts of multiple stressors, including lenging and generally limits replication, parameters relevant to climate change and point source pollutants is developed. This platform leverages (1) the high rate of purely diffusive gas transport in aerogels to produce well-defined centimeter-scale gas concentration gradients, (2) spatial light control, and (3) established automated liquid hanmaking it difficult to truly measure an effect—once the number of experimental treatments reaches the 100’s—typical of multiparameter studies.[5,7]. We demonstrate compatibility with multicellular organisms, Lemna gibba and Artemia salina
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