Abstract
Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes. Methods: The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data. Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups. Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes.
Highlights
Taking DNA straight from oral samples, without culture samples, is a fundamental principle of oral microbiome study
Read analysis We endeavoured to determine if the oral microbial community would reveal the different profiles of the six selected periopathogensin pooled samples collected from periodontitis subjects with (DP) and without (NPD) diabetes
The descriptive analysis prevented us from testing the directional relationship between diabetes and periodontitis. This is the first study in Indonesia to show that using the Nanopore MinION sequencing technology, we can investigate the presence of a consortium of red complex bacteria (P. gingivalis, T. forsythia, and T. denticola) that includes three genera (Aggregatibacter, Fusobacterium, and Veillonella) in periodontitis subjects with and without diabetes
Summary
Taking DNA straight from oral samples, without culture samples, is a fundamental principle of oral microbiome study. The advancement of sequencing technology has led to the mainstream in oral microbiology due to the increasing affordability and improvement in the speed of the sequencing process and quality of data obtained. In this context, MinION is the first sequencer available commercially that uses nanopore technology. The sequencing method provided by the MinION device does not rely on the synthesis of nucleotides For this reason, we decide to use this long-read nanopore sequencing device to identify the dominant bacteria in diabetic condition-related periodontitis. Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Aggregatibacter sp., which belongs to version 4 (revision)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
