Abstract
A photoetched matrix of indium tin oxide (ITO) on glass has been developed and tested as a tool to assist in the relocation and identification of individual neuronal cells in culture. The matrix is formed by 10–15 μm wide and 300 Å thick ITO lines which subdivide a 1-cm 2 area into 625 smaller squares. Each of the smaller squares measures 400 μm on a side and contains a photoetched two-letter “address”. The address code allows precise relocation of specific regions of a culture as well as verification of the identities of individual neurons selected for repeated observation. Marks at 50 μm intervals along the sides of the address squares permit quantitative analysis of morphological changes, cell migration, reaggregation, etc. The ITO is transparent and does not interfere with visualization of even fine details of cells with high power microscopy.
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