Abstract
A simple technique is described for the preparation of permanent stained smears of human E-rosetted lymphocytes. Smears of the cells suspended in foetal calf serum were drawn as thin strips on slides with a pen nib. Such smears, after Romanowsky staining, and incorporating latex-particle phagocytosis as a marker for non-lymphoid cells, show excellent rosette preservation and permit easy distinction and counting of rosetting lymphocytes, non-rosetting lymphocytes, monocytes and granulocytes. The percentages of rosette-forming lymphocytes estimated on such smears correspond closely to those obtained from counts made on viable cell suspensions.
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