Abstract
Almond is one of the most important nut crops in the world. The origin of almond is central and west Asia. About 30 wild species are identified in this region which represent a rich source of desirable characteristics useful in almond breeding programs. However, almonds express gametophytic self- and cross-incompatibility which affects breeding parent selection. Incompatibility in almond is controlled by a multi-allelic, single gene (S-locus). Recently chemical and molecular methods have been utilized to survey the S-alleles in almond cultivars and closely related species. The restriction fragment length polymorphism method using CAPs protocol was used to determin S-alleles in multiple accessions of wild almond and related Prunus species. Polymerase chain reactions were carried out using one set of degenerate primer pairs (EM-PC2consFD/EM-PC3consRD), and PCR products were digested by five restriction enzymes (TaqI, HinfI, DpnI, ApoI and AluI). The genotypes revealed different cutting paterns. ApoI and DpnI produced the most and the least bandings respectively. AluI, ApoI and HinfI digested the S7. ApoI was the only enzyme which could digest the S9. TaqI, ApoI and HinfI digested the S8 and S12.
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