Abstract
A paper chromatographic analysis of ten species of Tradescantia was made to determine if biochemical methods could be used to detect specific differences among the species and possible pathways of speciation. Two-dimensional chromatograms of extracts from roots, leaves, and flowers were examined, using ninhydrin and ultraviolet light. Ninhydrin-positive spots exhibited mostly quantitative differences. Ultraviolet-fluorescing spots from the three extracts yielded different patterns. The virginiana complex, encompassing seven of the ten species, could not be separated without accepting a one- or two-spot difference as being conclusive. The other three species could be separated with less difficulty. The relationships of the North American species, as determined by other workers, were supported by the results of this study.
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