Abstract
To find a novel human ion channel gene we have executed an extensive search by using a human genome draft sequencing data base. Here we report a novel two-pore domain K+ channel, TRESK (TWIK-related spinal cord K+ channel). TRESK is coded by 385 amino acids and shows low homology (19%) with previously characterized two-pore domain K+ channels. However, the most similar channel is TREK-2 (two-pore domain K+ channel), and TRESK also has two pore-forming domains and four transmembrane domains that are evolutionarily conserved in the two-pore domain K+ channel family. Moreover, we confirmed that TRESK is expressed in the spinal cord. Electrophysiological analysis demonstrated that TRESK induced outward rectification and functioned as a background K+ channel. Pharmacological analysis showed TRESK to be inhibited by previously reported K+ channel inhibitors Ba2+, propafenone, glyburide, lidocaine, quinine, quinidine, and triethanolamine. Functional analysis demonstrated TRESK to be inhibited by unsaturated free fatty acids such as arachidonic acid and docosahexaenoic acid. TRESK is also sensitive to extreme changes in extracellular and intracellular pH. These results indicate that TRESK is a novel two-pore domain K+ channel that may set the resting membrane potential of cells in the spinal cord.
Highlights
Channel), and TRESK has two pore-forming domains and four transmembrane domains that are evolutionarily conserved in the two-pore domain K؉ channel family
These results indicate that TRESK is a novel two-pore domain K؉ channel that may set the resting membrane potential of cells in the spinal cord
As described under “Experimental Procedures,” a series of rapid amplification of cDNA end studies using primers designed from this region resulted in identification of a full-length coding sequence (CDS) (1158 bases) with an open-reading frame encoding 385 amino acids (Fig. 1A)
Summary
Channel), and TRESK has two pore-forming domains and four transmembrane domains that are evolutionarily conserved in the two-pore domain K؉ channel family. These results indicate that TRESK is a novel two-pore domain K؉ channel that may set the resting membrane potential of cells in the spinal cord. Molecular Cloning of Human TRESK—The two-pore domain Kϩ channel-related sequence, accession number AL731557, was identified in the human draft genome sequencing data base of GenBankTM using a TBLASTN search for the amino acid sequence of human TWIK-1.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
