Abstract
Indole, as a signal molecule, is involved in multiple physiological behavior including biofilm formation, antibiotic resistance and virulence. In this study, we demonstrated that indole was involved in iron deficient and H2O2 stress response in Muricauda olearia Th120. Transcriptome analysis showed that totally 206 genes were regulated by exogenous indole. Besides, momL-suf gene cluster, consisting of quorum quenching enzyme coding gene momL and iron-sulfur biosynthetic genes suf, were involved in indole-induced stress response pathway. The result indicated that indole not only up-regulated momL-suf gene cluster, but also enhanced the MomL secretion and the growth rates of MomL-bearing strains in H2O2 stress and iron deficient culture conditions. Co-incubation of M. olearia Th120 and Pectobacterium carotovorum subsp. carotovorum under H2O2 condition revealed that M. olearia Th120 bearing MomL possessed an increased competitive advantage, whereas its competitor had a reduced survival. The phenomenon that quorum quenching enzyme is triggered by stress factor has been rarely reported. The study also opens a new clue to explore the indole function towards quorum quenching factor in bacteria.
Highlights
Quorum sensing (QS) is a population-dependent behavior that enables bacteria to communicate with each other and to regulate downstream gene expression in response to environmental changes[1,2,3,4,5]
Our experiments indicate that indole can improve the survival of M. olearia Th120 in the presence of H2O2
We focused on the study of whether suf of M. olearia Th120 was involved in indole-induced iron deficient and H2O2 stress response
Summary
Quorum sensing (QS) is a population-dependent behavior that enables bacteria to communicate with each other and to regulate downstream gene expression in response to environmental changes[1,2,3,4,5]. Many Gram-positive and Gram-negative bacteria encode a single copy of the tnaA gene in their chromosome and produce indole It plays diverse biological roles including biofilm formation, sporulation and virulence[10, 12,13,14]. MomL is located within the stress-response suf operon and is closely linked to sufB and sufC. This gene organization has not been previously reported in other organisms. We focused on the regulation mechanism of signal molecular indole towards the stress-response genes suf and QQ gene momL, and it firstly described the triggering of the expression of a QQ enzyme by stress factors
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