Abstract
A highly simple, rapid, sensitive and selective method is developed for spectrophotometric determination of gabapentin in pure form as well as in pharmaceutical formulations. The method is based on the formation of a yellow Schiff base derived from the condensation of gabapentin drug (1-amino methyl) cyclo hexane acetic acid and 2,5-dihydroxybenzaldehyde (DHBA) exhibiting a maximum absorbance at 445 nm. The composition, molar absorptivity and effect of different excipient have been determined spectrophotometrically. Under optimized experimental conditions, Beer’s law is obeyed in the concentration range 2.57 - 37.25 μg/ml. The method is validated with respect to accuracy, precision, limit of detection and limit of quantification. The Sandell sensitivity, correlation coefficient and regression equation are calculated. The equilibrium constant and free energy change using Benesi-Hildebrand plot are also determined. The Schiff base derived from condensation of gabapentin with DHBA is also synthesized and characterized. The condensation reaction mechanism has been proposed.
Highlights
Gabapentin (GBP) (1-(aminomethyl) cyclohexane acetic acid) is a new antiepileptic drug which is a structuralHow to cite this paper: Saleh, M.S., Youssef, A.K., Hashem, E.Y. and Abdel-Kader, D.A. (2014) A Novel Spectrophotometric Method for Determination of Gabapentin in Pharmaceutical Formulations Using 2,5-Dihydroxybenzaldehyde
Despite that the spectrophotometric techniques had been commonly applied for the determination of some other drugs [20], only few spectrophotometric methods were reported in literature for determination of GBP, based on the reaction of the primary amino group of GBP with ninhydrine reagent in organic solvent medium [21]-[23]
Other spectrophotometric methods involve the determination of GBP via formation of charge-transfer complexes with π-acceptors in pharmaceutical formulations [24]-[26]
Summary
Gabapentin (GBP) (1-(aminomethyl) cyclohexane acetic acid) is a new antiepileptic drug which is a structuralHow to cite this paper: Saleh, M.S., Youssef, A.K., Hashem, E.Y. and Abdel-Kader, D.A. (2014) A Novel Spectrophotometric Method for Determination of Gabapentin in Pharmaceutical Formulations Using 2,5-Dihydroxybenzaldehyde. Gabapentin (GBP) (1-(aminomethyl) cyclohexane acetic acid) is a new antiepileptic drug which is a structural. (2014) A Novel Spectrophotometric Method for Determination of Gabapentin in Pharmaceutical Formulations Using 2,5-Dihydroxybenzaldehyde. The reported analytical methods for GBP determination include high performance liquid chromatography (HPLC) [3]-[14], spectrofluorometry [15] [16], gas chromatography [17]-[19]. Despite that the spectrophotometric techniques had been commonly applied for the determination of some other drugs [20], only few spectrophotometric methods were reported in literature for determination of GBP, based on the reaction of the primary amino group of GBP with ninhydrine reagent in organic solvent medium [21]-[23]. Other spectrophotometric methods involve the determination of GBP via formation of charge-transfer complexes with π-acceptors in pharmaceutical formulations [24]-[26]
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