Abstract
Intramuscular fat (IMF) is one of the most important economic characteristics of meat quality. In this study, the IMF content of 921 Hu sheep was determined by Soxhlet extraction method. RT-qPCR was used to detect expression profiles of LIPE in different tissues and longissimus dorsi (LD) muscle with low, medium and high IMF content. Pooled-DNA sequencing and improved multiple-temperature ligase detection reaction (iMLDR) methods were used to screen single nucleotide polymorphisms (SNPs) within LIPE. The results showed that the IMF content of Hu sheep ranged from 1.34% to 11.26%, and it was more beneficial to IMF deposition in the summer than in the winter (P < 0.0001). The expression level of LIPE in perirenal fat was significantly higher than that in other tissues, and showed a decreasing trend with the increase of IMF content. A total of ten SNPs were identified within LIPE. Of which, one novel SNP in intron 4 (g.4819 A > G) was significantly correlated with IMF content (P = 0.001). These results indicate that g.4819 A > G in LIPE can be used as a potential genetic marker to select Hu sheep with desirable IMF content.
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