Abstract
In eukaryotes, the expression of most genes is regulated by alternative pre-mRNA splicing (AS), through which introns are selectively removed to generate multiple transcript variants from a single gene. Serine/arginine-rich (SR) proteins are major modulators of alternative splicing, a key generator of proteomic diversity and flexible means of regulating gene expression likely to be crucial in plant environmental responses. In this work, we aimed to isolate a gene of SR from Gardenia jasminoides. A G. jasminoides fruit cDNA library was constructed, and the GjSR45a cDNA was isolated from the cDNA library by sequencing method. Sequence analysis revealed an open reading frame encoding a protein of 237 amino acid residues. Alignment of the amino acid sequence with AtSR45a and Human Tra2b revealed that the protein contains RRMs (RNA recognition motifs) motif and two RS (arginine/serine-rich) domains at the N- and the C-terminus, which are modular domains characteristic of RS-rich splicing factor. A three-dimensional dimmer model of GjSR45a was built. Comparison of the 3D structure with HomTra2b revealed highly similarity. The results suggest that GjSR45a is a SR45a like protein mainly responsible for alternative splicing.
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