Abstract
The marine algal toxin palytoxin (PLTX) and its analogues are some of the most toxic marine compounds. Their accumulation in edible marine organisms and entrance into the food chain represent their main concerns for human health. Indeed, several fatal human poisonings attributed to these compounds have been recorded in tropical and subtropical areas. Due to the increasing occurrence of PLTX in temperate areas such as the Mediterranean Sea, the European Food Safety Authority (EFSA) has suggested a maximum limit of 30 µg PLTX/kg in shellfish meat, and has recommended the development of rapid, specific, and sensitive methods for detection and quantitation of PLTX in seafood. Thus, a novel, sensitive cell-based ELISA was developed and characterized for PLTX quantitation in mussels. The estimated limits of detection (LOD) and quantitation (LOQ) were 1.2 × 10−11 M (32.2 pg/mL) and 2.8 × 10−11 M (75.0 pg/mL), respectively, with good accuracy (bias = 2.5%) and repeatability (15% and 9% interday and intraday relative standard deviation of repeatability (RSDr), respectively). Minimal interference of 80% aqueous methanol extract allows PLTX quantitation in mussels at concentrations lower than the maximum limit suggested by EFSA, with an LOQ of 9.1 µg PLTX equivalent/kg mussel meat. Given its high sensitivity and specificity, the cell-based ELISA should be considered a suitable method for PLTX quantitation.
Highlights
Palytoxin (PLTX), a complex marine poly-ol toxin, is one of the most toxic natural compounds.The discovery of PLTX dates back to the 1960s, when, in a tide pool of Hana Bay (Maui Island, Hawaii), Prof
There is a need to develop new detection methods able to quantify PLTXs in seafood at concentrations lower than the maximum limit suggested by European Food Safety Authority (EFSA) (30 μg PLTX/kg of shellfish meat) [35]
We developed a cell-based immunoenzymatic assay that combines the high binding potency of PLTX with the sensitivity and specificity provided by anti-PLTX antibody detection and evaluated it for its ability to accurately quantify PLTX in mussels
Summary
Palytoxin (PLTX), a complex marine poly-ol toxin, is one of the most toxic natural compounds. Signs and symptoms in the respiratory tract, including dyspnea associated with fever >38 ◦ C, as well as conjunctivitis and dermatitis have been reported [13,14,24,27,30,32,33] In these areas, Ostreopsis has been recorded since the early 1970s [34], and in the last decade PLTXs have been detected both in microalgae and in edible marine organisms, but no foodborne poisonings attributed to these toxins have yet been documented. Ostreopsis has been recorded since the early 1970s [34], and in the last decade PLTXs have been detected both in microalgae and in edible marine organisms, but no foodborne poisonings attributed to these toxins have yet been documented Despite their high toxicity, PLTXs are not regulated as seafood or environmental contaminants. This novel method was further characterized for its suitability to quantify the toxin in mussels
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
