Abstract
Objective: Melanoma associated antigens-A (MAGE-A) expression is highly specific to cancer cells. Thus, they can be the most suitable targets for the diagnosis of malignancy. The aim of this study was to evaluate the sensitivity of multiple MAGE-A expression analysis for the diagnosis of oral squamous cell carcinoma (OSCC).Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of otherwise healthy volunteers were examined for the expression of 10 different single antigens out of 12 different MAGE-A subtypes by highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) methods. The results were correlated to clinicopathological parameters of tumor samples.Results: Expression of MAGE-A was restricted to OSCC. The expression frequency of single antigen was between 10% and 55%. However, expression rate was increased up to 93% by the elevated number of genes examined. A significant correlation was found between the expression of MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A detection has also correlated to the incidence of lymph node metastasis, grading and advanced clinical stages.Conclusions: Analysis of multiple MAGE-A expression is more sensitive than the analysis of a single MAGE-A for the diagnostic evaluation of OSCC. Multiple MAGE-A expression analysis may be a very sensitive method to be used for the diagnosis even in the early stage of OSCC.
Highlights
Oral squamous cell carcinoma (OSCC) is the 6th most frequent occurring cancer world wide [1]
Expression of 10 Melanoma associated antigens-A (MAGE-A) was analyzed all together in number of OSSC samples in order to increase the sensitivity for the accuracy
Elevated number of MAGE-A analysis does increase gradually the detection of sensitivity for OSCC cells when a certain combination was taken into account, especially when the amount of sample is not sufficient to perform analysis for each markers separately
Summary
Oral squamous cell carcinoma (OSCC) is the 6th most frequent occurring cancer world wide [1]. Despite the extensive researches on treatment modalities for OSCC five year survival rate has been reported to be approximately 50% and has not been improved over the last four decades [2,3]. The major contributing factors for un-improvement in the survival rate might be the late diagnosis of primary tumors and the high incidence of local recurrences due to occurrence of occult cancer cells in tumor margins [4,5]. OSCC is known to exhibit “field cancerization” which may result in a second primary tumor [6]. Early dissemination of tumor cells into bone marrow or peripheral blood during the surgery may cause recurrence and metastasis, which is usually overlooked. To increase the survival rate of patients with OSCC, highly sensitive and specific molecular diagnos-
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
