Abstract
Indocyanine green (ICG) accumulates only in hepatocytes and their malignant counterpart, hepatocellular carcinoma (HCC). We have developed ICG-conjugated anti-cancer drugs and noted their significant accumulation in HCC cells both in vitro and in vivo. ICG-conjugated gemcitabine was less toxic to normal cells and it had superior anti-tumor action compared to gemcitabine alone in a subcutaneous tumor xenograft. ICG conjugation can provide a novel fluorescent drug delivery system for treatment of liver cancer and this system can be used to both diagnose and treat HCC.
Highlights
Hepatocellular carcinoma (HCC) is the second leading cause of cancer mortality worldwide[1]
After hepatocellular carcinoma (HCC) cells were incubated for 24 h, a fluorescence microscope revealed indocyanine green (ICG)-Gem accumulation in the cytosol of both HCC cell lines, HuH-7 and HepG2, and this accumulation was not noted in colon cancer cell (HCT116) (Fig. 1B)
ICG fluorescence was detected in the nuclei of HCC cells (Fig. 1B right lower panel) it was weaker than that in cytosol
Summary
Cultured HuH-7 cells were harvested in tubes and resuspended in conditional medium containing 10% FBS after they were washed with PBS. Fluorescence of ICG conjugates in cultured cells was detected using a fluorescence microscope (Hamamatsu Photonics, Hamamatsu, Japan). Cultured HuH-7, HepG2 and HUVEC cells were harvested in tubes and resuspended in conditional medium containing 10% FBS after they were washed with PBS. The cells were seeded in triplicate in 96-well plates at a density of 5 × 103 cells in 200 μL and incubated with a series of concentrations of ICG-Gem for 48 h at 37 °C in a 5% CO2 atmosphere. Cultured HuH-7 cells were harvested in tubes and resuspended in serum-free medium after they were washed with PBS.
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