Abstract
An ESIPT-based fluorescent probe (Probe 1) with a large Stokes shift (202 nm) for the sensing of biothiols has been developed based on cyanobiphenyl benzothiazole derivative exhibiting larger Stokes shift than 2-(benzothiazol-2-yl)phenol. This probe with 2,4-dinitrobenzenesulfonate (DNBS) as a highly thiol-selective group was constructed based on the combination of excited state intramolecular proton transfer (ESIPT) and photoinduced electron transfer (PET) mechanisms. Upon the treatment with biothiols, this probe produced a remarkable fluorescence enhancement at 482 nm. The detect limits for Cys, GSH and Hcy was calculated to be as low as 2.0 × 10−8 M, 1.7 × 10−7 M and 1.2 × 10−7 M, respectively (based on S/N = 3). Importantly, application of this probe was successfully demonstrated by imaging thiols in living NCI-H226 cells.
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