Abstract
Exosomes have critical role in regulating the tumor development and progression and resistance following antiangiogenesis therapies (AATs). Exosomes could be released by both tumor cells and surrounding endothelial cells (ECs). Here, we describe the methods to explore the cargo transfer between tumor cells and ECs by a novel four-compartment co-culture methods and to investigate the effect of tumor cells on angiogenic ability of ECs by Transwell co-culture methods.
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