Abstract
Kaempferol-3-O-[6Glc-O-feruloyl]-β-D-glucopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside (1) was isolated from the aerial parts of Brunfelsia grandiflora ssp. grandiflora. The structure of this novel flavonol glycoside was established by fast atom bombardment-mass spectrometry and by means of NMR spectroscopy at 14T, using two-dimensional NMR experiments with pulsed-field gradients for coherence selection. The presence of the constituent aglycones and sugar units of 1 was confirmed independently by acid hydrolysis. Copyright © 2000 John Wiley & Sons, Ltd.
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