A note on the biodiversity of Ghadamgah spring–stream system in Fars province, southwest Iran

Interbasin water transfer has become a means by which countries around the world solve the problem of water shortage. Moreover, interbasin water transfers may also stimulate stronger controls over water pollution control in water‐receiving areas. However, most existing literature fails to examine the impacts of interbasin water transfers on water consumption and pollution from the perspective of the complex entanglement between human activities and environmental change. In order to respond to this research gap, this study establishes an improved human–environment model that considers the impacts of interbasin water transfer, and uses data about China's South–North Water Transfer Project to empirically study the temporal and spatial effects of interbasin water transfers. The results show that interbasin water transfer not only fails to improve water consumption in water‐receiving cities but also further restricts residents' water use due to the high water price caused by this project. Furthermore, interbasin water transfers also fail to reduce water pollution in water‐receiving cities—levels of water pollution depend more on external environmental policies. These results have some implications for understanding the interbasin water transfers in authoritarian states: although interbasin water transfers do not affect water consumption and pollution, authoritarian states can maintain and consolidate their political legitimacy by gaining public trust through interbasin water transfers.

The incidence and mortality rates of cancer are increasing worldwide, particularly in the developing countries. Valid data are needed for measuring the cancer burden and making appropriate decisions toward cancer control. We evaluated the completeness of death registry with regard to cancer death in Fars Province, I. R. of Iran. We used data from three sources in Fars Province, including the national death registry (source 1), the follow-up data from the pathology-based cancer registry (source 2) and hospital based records (source 3) during 2004 - 2006. We used the capture-recapture method and estimated underestimation and the true age standardized mortality rate (ASMR) for cancer. We used log-linear (LL) modeling for statistical analysis. We observed 1941, 480, and 355 cancer deaths in sources 1, 2 and 3, respectively. After data linkage, we estimated that mortality registry had about 40% underestimation for cancer death. After adjustment for this underestimation rate, the ASMR of cancer in the Fars Province for all cancer types increased from 44.8 per 100,000 (95% CI: 42.8 - 46.7) to 76.3 per 100,000 (95% CI: 73.3 - 78.9), accounting for 3309 (95% CI: 3151 - 3293) cancer deaths annually. The mortality rate of cancer is considerably higher than the rates reported by the routine registry in Iran. Improvement in the validity and completeness of the mortality registry is needed to estimate the true mortality rate caused by cancer in Iran.

The revised checklist of reptiles for Fars Province, southern Iran, comprises of 63 species in 41 genera, 17 families and two orders (Squamata and Testudines). The most diverse suborder is the Sauria with 38 species or 60.31% of the herpetofauna, followed by Serpentes (23 species, 36.5%) and the Testudines (2 species, 3.17%). The most diverse families are the Colubridae and Gekkonidae with 15 and 14 species (23.8% and 22.2%), respectively, followed by Agamidae (9 species, 14.3%), Lacertidae and Scincidae each with five species or 7.9% of the herpetofauna, and Viperidae (four species, 6.35%). Eleven families have only one species each. The Fars Province is the type locality of several endemic species, as a new species has recently been described as well as several newly recorded species.

The common fig (Ficus carica L.) is cultivated for its fruits in warm and temperate parts of Europe, Mediterranean and Iran. It is the main garden product in Estahban township, Fars province, southern Iran and area under cultivation of figs reaches 22000 hectares. There are some experiments conducted on fauna of fig mite. The mite Pronematus ubiquitus has been reported as faunistic of fig orchards in Khozestan and Saveh (Kamali & Shishehbor 1989; Baradaran & Arbabi 2002). Also, Rhycaphytoptus ficifolia was reported as pest in figs in Iran and other part of world (Arbabi et al. 1994; Abou-Awad et al. 1999). Literatures show that there are few studies regarding fauna of fig mites in Iran, therefore this study was performed to evaluate this mite in fig trees of Estahban. Estahban is located in the latitude 29o 07 N and longitude 54o 02 E and altitude of 1767 m above sea level. During 2010–11, in the course of a faunistic survey of mites associated with fig mite in Estahban, soil, leaves and fruits samples were taken from fig orchards. Samples were transferred into the Acarological laboratory of the Department of Plant Protection, College of Agriculture, Shiraz University. Mites were extracted using a Berlese funnel or separated under a steriomicroscope. Specimens were removed and cleared in lactophenol and mounted in Hoyer's medium on glass microscopic slides for identification. Specimens are deposited in the Acarological Collection of the Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran and Acarological Collection, The Acarological Society of Iran, Kraj, Iran.

Recently, Leishmania infantum has increasingly been detected in stray cats in endemic regions of the world. Cats have been considered playing a role in the epidemiology of visceral leishmaniosis, an endemic zoonosis in Iran. The studies concerning feline leishmaniosis (FeL) allow the hypothesis that cats can be considered as potential reservoirs. The investigations on Leishmania infection in cats are very few in Iran and therefore we aimed to assess the L. infantum infection in stray cats and its possible role in transmission of the disease to human by direct agglutination test (DAT), ELISA, nested-PCR and confirmation via sequencing and phylogenetic analysis in Fars province, Iran. Whole blood samples were obtained from 174 stray cats. Anti-Leishmania antibodies were detected in the sera using DAT and ELISA. DNA was extracted from the buffy coat of each subject and PCR amplified, targeting Leishmania kDNA gene. PCR results were confirmed by sequence analysis. Prevalence of clinical signs in positive cats was 19.0%. Anti-Leishmania antibodies with different titers were detected in 48 (27.59%) and leishmanial DNA in 36 (20.69%) of the cats. The sequencing of PCR-positive cats revealed the parasite as L. infantum. A high seroprevalence of L. infantum was revealed, with higher levels in males, adult cats, and those living in rural districts and southern zones. Despite the reservoir task of cats in nature is still ambiguous, the high serological and molecular detection of L. infantum in stray cats indicates that cats are regularly bitten by infected sand flies in Fars province, southern Iran, and may have a potential reservoir role in the maintenance of L. infantum in the endemic areas of zoonotic visceral leishmaniosis in Iran. Anyway, Leishmania infection must be appraised in the differential diagnosis of cutaneous or systemic clinical signs in cats.

Featured Collection Introduction: Severe Sustained Drought Revisited: Managing the Colorado River System in Times of Water Shortage 25 Years Later — Part I

Background. Toxoplasma gondii is an intracellular protozoan parasite responsible for systemic disease in a wide range of warm-blooded animals. The current study is aimed at evaluating the prevalence of Toxoplasma infection in dogs, using serological and molecular methods in rural areas in Kazeroun Township, Fars province, southern Iran. Methods. Blood samples were obtained from 60 clinically healthy dogs with an age range of 1 to 7 years in three rural areas of Fars province, southern Iran. Sera and buffy coats were used to assess the T. gondii infection using both modified agglutination test (MAT) and real-time PCR. Results. Antibodies against T. gondii were detected in 5 out of 60 (8.3%) dogs by the MAT method, and T. gondii DNA was detected in 17 out of 60 (28.3%) studied animals. There was no significant association between sex and seropositivity to Toxoplasma (p > 0.05). Fair agreement (kappa = 0.27) was seen between molecular and serological findings where three dogs with positive serological results had a positive molecular test. Conclusion. Findings of the present study show a relatively high prevalence of T. gondii infection in dogs in rural areas in Fars province, southern Iran. Finding the parasite genotype in dogs deserves further study.

Background:Dogs, as the definitive host of Neospora caninum, are important in the epidemiology of this parasitic infection. We aimed to determine the prevalence of N. caninum infection in a dog population from a rural setting in Fars Province, Southern Iran, using a combination of molecular and serological techniques.Methods:This cross-sectional study was carried out in Nov 2018 in three rural districts, Sar Mashhad, HosseinAbad, and Tolesaman located in Kazeroun Township in Fars province, southern Iran. Blood samples were taken from 60 stray and household dogs. Dogs’ sera were tested for antibodies against N. caninum, using a Neospora-Modified Agglutination Test. Moreover, dogs’ buffy coats were tested for Neospora DNA, using a molecular method.Results:Anti-Neospora antibodies were detected in sera of 4 out of 60 dogs, corresponding to a seroprevalence rate of 6.7%. Out of 25 female dogs, 1 was seropositive and of 35 males, 3 were seropositive, yet the differences were not statistically significant. The infection was more prevalent in adult dogs (> 12 months), nevertheless, the differences between age and Neospora seropositivity was not statistically significant. N. caninum DNA was not detected in the buffy coat of any of the studied dogs.Conclusion:Findings of the study indicate that N. caninum is a common infection in dogs in rural areas of Fars province in southern Iran. The infected dogs might be a potentially important source of N. caninum infection to livestock in the area.

Barbus sublimus, described by Coad and Najafpour (1997) as a new species, was retrieved first from the Aala Rood, a river in Khuzestan province, southwestern Iran. In our latest sampling a population of this species was found, for the first time, in Fahlian Rood, a river in a different water basin, in Fars province, Southwest of Iran, where no specimen of the species was collected in previous samplings at this location. The detail of taxonomic work done on the specimens captured, species phylogeny, and the possibilities of its previous absence and then presence are described and discussed.

Some parts of the U.S. have strained or insufficient local water supplies to meet the demands of population, industry and agriculture located in the region. Some areas with insufficient water supply have long implemented measures to address the shortfall through transferring water from other basins. New York City obtains almost 97% of its water and Los Angeles over 90% from interbasin transfers (IBTs).<br>With climate change affecting precipitation and temperature patterns across the U.S., coupled with growth in population and the economy leading to changes in demand, planning for risks to water supplies is critical to ensuring continued supply of water for all U.S. regions. Assessment of areas of high and low water risk can provide insights into potential changes in availability for existing supply, and aid in decision making for mitigating forecasted risks to local water supply. Implementation of IBTs historically has been one approach for addressing water supply risks.<br>The overarching goal of this research was to examine the role of IBTs for water resource supply and management in the U.S. Specific objectives were as follows:<br>1. Quantify the number of IBTs that exist at a defined hydrologic unit code (HUC) level in the U.S. and examine the distribution of IBTs and potential causes associated with any observed clustering of IBTs. <br>2. Characterize and classify IBTs, and examine the development drivers for a subset of IBTs in the U.S through sampling in different climate regions of the U.S.<br>3. Examine the water risks in the U.S. by county, considering both current and future conditions and accounting for natural water importation through streams and rivers, and consider the role of IBTs in mitigating these risks.<br>As part of the first objective, the definition of what constitutes a “basin” was required to assess man-made transfers that cross those basin boundaries. There are several definitions utilized by different states, with no federal definition. The most recent inventory of IBTs was conducted by the USGS in 1985 and 1986 using the HUC4 level. To build a new inventory of IBTs in the U.S., the National Hydrography Dataset (NHD) was utilized, combined with the Watershed Boundary Dataset (WBD). Man-made transfers across basin boundaries at the HUC6 level were considered to be interbasin. Geographic Information Systems (GIS) analysis showed that as of 2016 there were 2,161 IBTs crossing HUC6 boundaries in the U.S. These were located across the country, although over 50% of those identified were located in Florida, Texas or North Carolina. Some clustering of IBTs was observed in various states and analysis of the clustering suggested a variety of reasons for IBT construction, including population, drainage and agricultural factors. However, the flow volumes associated with the IBTs identified could not be evaluated due to a lack of available data at both the state and federal level.<br>The second objective expanded upon this analysis, examining a subset of 109 (5%) of the identified IBT reaches within the various climate regions of the U.S. To characterize and classify the IBTs each was labeled as being near irrigated agricultural land, near cities, or rural for those not near either cities or irrigated land. IBTs in proximity to both cities and irrigated agricultural lands were given the designation city+irrigated agriculture. Selection of IBTs for this analysis was based on the approximate proportional distribution of the total number of IBTs within each climate region and included representation of IBT clusters identified as part of the first objective. The results of the analysis showed that there have been four major drivers behind the construction of IBTs in the U.S.: irrigation for agriculture, municipal and industrial water supply, commercial shipping or navigation, and drainage or flood management. The most common factor for IBT construction has been to enable drainage or flood management. IBT development for agricultural needs has also been prevalent. The majority of IBTs examined were constructed between 1880 and 1980, with peaks in construction occurring between 1900-1910 and 1960-1970. The case studies examined showed that drivers of IBT development evolved through history, reflecting the changes in U.S. and regional economies, populations and needs.<br>To examine the risks associated with the U.S. water supply a new Water Risk Index (WRI) was developed, building upon and advancing a prior risk analysis developed by Roy et al. (2012). The Roy et al. work utilized risk factors that focused upon local precipitation, demand and evapotranspiration, without examining the natural flow of water between counties. To produce the WRI the analysis utilized the 2015 USGS Water Use Report data and projected water use in 2050, assuming only municipal and domestic water demand and thermoelectric power water withdrawal demand would change over time as per Roy et al. (2012). To calculate the flow volumes for each county the Water Supply Sustainability Index (WaSSI) developed by the USDA Forest Service (Sun, 2008) was used. The WaSSI model allowed for the analysis to include changes in climate and related hydrology as well as the evolving water demand. The WRI calculated water supply risk for each county in the contiguous U.S. The WRI calculation includes comparisons of water withdrawal to local flow volume, the drought susceptibility during summer for both the present and future, the projected growth in water demand, and the proportion of groundwater use relative to total water demand. This risk index provides a scaled value system that provides context to each individual risk factor included. The results of this showed that while some counties are regarded as high or very high risk, there are significantly fewer than those identified by the Roy et al. (2012) analysis. A maximum of 36 counties were identified as high or very high risk within the scenarios examined as part of the WRI analysis, in comparison to over 400 in the previous analysis. The highest risk areas are located in the west, with most counties determined to be at very high risk located in California. Most of the counties with negligible risk are located in Montana and Wyoming, as well as Colorado west of the continental divide.<br>This research provides insights into locations within the U.S. that may have high risks to their water supplies, and into the role that current or potential IBTs can have to mitigate those risks. In addition, the methods developed can help support planners to identify low risk locations to examine for their potential to support IBT water supply solutions while accounting for the downstream impacts such diversions may cause. To ensure that the U.S. maintains a consistent and secure water supply all options must be considered for their viability, including the potential for moving water from where it is plentiful to areas it is not.<br>

Background: Toxoplasma gondii is a zoonotic protozoan that threatens the health of the people infected with human immunodeficiency virus (HIV). HIV-positive subjects are at risk of cerebral toxoplasmosis. Objectives: The current study was designed to find out the seroprevalence of toxoplasmosis in HIV-infected individuals in Fars province, in the South of Iran. Methods: This cross-sectional study was performed on 251 HIV-infected individuals referred to Shiraz HIV/AIDS Research Center in Fars province, Southern Iran, in 2016. Blood samples (5 mL) were collected from each individual and sera as well as buffy coat were isolated from each sample. Demographic and HIV-associated data were obtained from the patients’ medical records. Anti-Toxoplasma antibodies (both IgG and IgM) were determined by ELISA, using a commercial kit. In addition, PCR, targeting a 529 bp gene of T. gondii was performed on the patients’ buffy coats for detection of Toxoplasma DNA. Results: Anti-Toxoplasma antibodies were detected in the sera of 42 out of 251 (16.7%) HIV infected patients. Of these, 39 cases (15.5%) were seropositive only for IgG and 3 (1.2%) were positive only for IgM. Seropositive subjects mainly belonged to 40 - 49 year age group. None of the subjects were positive for Toxoplasma DNA when evaluated by the PCR. No significant associations were found between Toxoplasma seropositivity and gender, age, and CD4+ cell level (P > 0.05). Conclusions: Findings of this study demonstrated a significant rate of seroprevalence of Toxoplasma in HIV infected subjects in Fars province, Southern Iran. The seropositive cases are at risk of Toxoplasma reactivation and subsequent cerebral encephalitis. Treatment and also prevention of toxoplasmosis in HIV positive people should be considered.

In the Koto Panjang Dam, Riau there are more than 1582fish floating cages present. Fish feed spilled from the cage is believed to affect the diet of wild endemic fishes inhabit the dam. To understand the effect of the cage on the diet of the endemic, a study has been conducted on May to September 2013. There were 5 sampling sites, 2 sites located in the natural area that there is no cage (St 1Na and St2 Na) and the others (3 Ka, 4 Ka and 5 Ka) were in the area around the cage. Fishes were sampled hourly, for a 24 hour period and their stomach content was analyzed. Results shown that there were 31 species of endemic fishes such as Puntius schwanenfeldii, Macrones nemurus, Hampala bimaculata, Osteochillus hasselti, Rasbora vaillanti, Tynnichtys vaillanti, Ophiocephalus melanosoma, Chela oxygastroides, Cyclocheilichthys heteronema, Notopterus chilata, Puntius bramoidesandCyclocheilichtys apogon. Among these fishes, however, only P. schwanenfeldii that greatly affected by the presence of the aquaculture activities as the stomach of fish living around the cage was filled with fish feed pellets (more than 90%). The fish living in the area with no cage, however, filled with animal and plant remains. This fact indicates that the P. schwanenfeldii is an opportunist fish that is able to change their diet into fish feed pellets. Based on data obtained, it can be concluded that the presence of fish floating cage aquaculture activities affect certain fish species only. Key words: Fish floating cage, Puntius schwanenfeldii, Koto Panjang Dam, endemic fish, fish feed pellet

Kala-azar (visceral leishmaniasis [VL]) is endemic in southern Iran. We retrospectively evaluated 367 infants and children suffering from VL at hospitals affiliated to the Shiraz University of Medical Sciences in Fars Province, southwest Iran). Seasonal variations were observed with more cases presenting in late winter, spring and fewer in summer. The predominant clinical features in these patients were chronic fever, pallor, weight loss, abdominal distention and hepatosplenomegaly. Lymphadenopathy was less common. Common laboratory abnormalities included anaemia, leukopenia, thrombocytopenia, hypoalbuminaemia and hypergammaglobulinaemia. Liver function tests were deranged in two-thirds of the patients. The immunofluorescence antibody test was positive in all patients and all of them had a positive bone marrow smear or a culture for Leishmania donovani. Patients responded well to glucantim therapy with a cure rate of 96.7%. Relapse was observed in 8.2% (30). Mortality in this series was 7.3%. Twenty patients died during their therapy period. Jaundice and grossly deranged liver function tests were found to be bad prognostic signs.

Congenital hypothyroidism (CH) may lead to irreversible mental retardation. To prevent the complication, screening was conducted routinely for all neonates in Iran. This study aimed to evaluate the appropriateness of CH screening programs in Fars province, southern Iran. This retrospective study (February-May, 2017) was conducted in the Health System Research Center of Shiraz University of Medical Sciences (SUMS). The data were obtained from the non-communicable diseases’ unit of SUMS regarding congenital hypothyroidism screening of all neonates born in Fars province from 2005 to 2015. We evaluated the coverage of CH screening, the incidence rate of CH, the percentage of the neonates screened for CH in ideal time, and neonates with CH treated in appropriate time. Descriptive statistics were used to determine the indices. To compare the incidence rate of boys and girls, we used Chi-square test. The coverage of CH screening in Fars Province increased from 50.0% in 2005 to 99.7% in 2015. Furthermore, the percentage of the neonates screened in the ideal time and the neonates with CH treated in an appropriate time increased from 26.0% and 78.0% in 2005 to 86.6% and 99.7% in 2015, respectively. In the evaluated period, the coverage of CH screening in Fars province (98.1%) was higher than that of the other provinces of Iran (83.0%). The study showed good screening coverage in Fars Province from 2005 to 2015. The coverage was considerably better than other provinces of Iran and improved during the study period. Also, over the course of time, more neonates were screened in an ideal time and treated in the appropriate time.

Cystic echinococcosis (CE) is a common zoonotic infection in all provinces of Iran, especially in areas where people live on agriculture and animal husbandry. The current cross-sectional population-based study is aimed at determining the seroprevalence of CE in Kavar County, Fars province, southern Iran. Blood samples were collected from 1500 individuals (749 males and 751 females). Anti-hydatid cyst antibodies were detected, using a recombinant B8/1 antigen of Echinococcus graunlosus in an ELISA system. Multivariate logistic regression analysis was used to find out the independent risk factor for CE seropositivity. Anti-hydatid cyst antibodies were detected in the sera of 131 out of 1500 participants, corresponding to a seroprevalence rate of 8.73%. With a statistically significant difference (p < 0.05), the seroprevalence of hydatid cyst in males and females was 5% and 7%, respectively. Differences in the seropositivity of hydatid cysts were also statistically significant by occupation (p < 0.05). The seroprevalence of hydatid cyst was significantly higher in the age group of 35-45 years than in other age groups (p < 0.05). Multivariate logistic regression showed that only age was associated with seropositivity to CE (OR = 2.23, 95% CI: 1.33-3.72). Moreover, there was a statistically significant association between seropositivity to hydatid cysts and underlying diseases such as hypertension (p = 0.049) and fatty liver (p = 0.048). The findings of this study indicate that the seroprevalence rate of CE is relatively high in the Fars province, southern Iran, and this high rate of infection is mainly linked to people's jobs and lifestyles.