Abstract
Abstract When human red cell suspensions were incubated with [14C1] glucose a substantial fraction of the incorporated radioactivity was found in phosphate compounds. If some of these phosphates are precursors of 14CO2 then an error is introduced into the conventional calculation of the proportion of glucose metabolised by the pentose phosphate pathway. The existence of this error was demonstrated and its magnitude was assessed by studying the effects of the subsequent addition of unlabelled glucose to the reaction mixtures in either the absence or the presence of methylene blue.
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