Abstract
Abstractmagnified imageA novel regeneration system for oxidized nicotinamide cofactors (NAD+ and NADP+) is presented. By combining 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulphonic acid (ABTS)‐catalyzed oxidation of NAD(P)H with laccase‐catalyzed utilization of molecular oxygen as terminal oxidant, a simple chemo‐enzymatic NAD(P)+ regeneration method is achieved. Thus, the advantages of both worlds, chemical oxidation of reduced nicotinamide cofactors and laccase‐catalyzed utilization of oxygen from air are combined in a simple and generally applicable new approach for biooxidation catalysis. This new application of the well‐known laccase‐mediator system (LMS) is successfully used to promote alcohol dehydrogenase‐catalyzed oxidation reactions of primary and secondary alcohols. Already under non‐optimized conditions, high turnover numbers of >300 and >16000 were obtained for the nicotinamide cofactor and ABTS, respectively. In this communication, we present the proof‐of‐principle and initial characterization of the proposed new regeneration system.
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