Abstract

A new phase for two-photon microscopy

Highlights

  • For all of its advantages, it is not without its limitations; for neuroscience, perhaps chief among these is the time it takes to collect a large image over a field of view containing many neurons

  • Conventional two-photon microscopy assembles an image by scanning a single laser spot over the sample

  • The second application was to image activity, via a calcium indicator, simultaneously in multiple neurons at speeds ranging from 15-60 Hz

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Summary

Introduction

A., Contreras, D., Steriade, M., Sejnowski, T. Ln vitro, and computational analysis of dendritic calcium currents in thalamic reticular neurons, J. Subcellular distribution of low-voltage activated T-type Ca(2+) channel subunits (Ca(v)3.1 and Ca(v)3.3) in reticular thalamic neurons of the cat.

Results
Conclusion

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