Abstract
In the present study, ergosterol peroxide and ergosterol were isolated for the first time from fresh fruit bodies of Hygrophoropsis aurantiaca (False Chanterelle). The substances were characterized mainly by spectroscopic methods (1H-NMR, 13C-NMR, DEPT-45, DEPT-90, DEPT-135, 2D-NMR). In our study, a new specific thin layer chromatographic method was developed for determination of ergosterol and ergosterol peroxide in H. aurantiaca extract. The method is based on the separation of n-hexane extract on silica gel (Silica Gel G) TLC plates using the optimized solvent system toluene/ethyl acetate (3:1; v/v). The main advantages of the developed method are the simplicity of operation and the low cost. The in vitro study results revealed the antiproliferative properties of ergosterol peroxide against LS180 human colon cancer cells. The described effect was attributed both to altered mitochondrial activity and decreased DNA synthesis. Additionally, in the same concentration range the investigated compound was not toxic to CCD 841 CoTr human colon epithelial cells. The present study suggests that fruit bodies of H. aurantiaca have great potential for producing substances and extracts with potential applications in medicine.
Highlights
Wild mushrooms have recently gained much attention as important sources of chemically interesting and biologically active secondary metabolites for the development of new pharmaceutical agents
The method is based on the separation of n-hexane extract on silica gel (Silica Gel G) Thin layer chromatography (TLC) plates using the optimized solvent system toluen/ethyl acetate (3:1; v/v)
Compound cytotoxicity was by measured of Results areResults presented as the mean of 4 ̆measurements
Summary
Wild mushrooms have recently gained much attention as important sources of chemically interesting and biologically active secondary metabolites for the development of new pharmaceutical agents. Mushrooms have been reported to have hypotensive, anticholesterolemic, hypoglycemic, antiinflammatory, antibacterial, antiviral, and antifungal properties [1,2,3]. America mushroom commonly known as the False Chanterelle, distributed in Europe and North America in in summer andand autumn. This compound antibacterial activity, in NRK-49F kidney(afibroblast line). Their structures were established using (Figure 1) were isolated from ethanolic extracts of H. aurantiaca The antiproliferative activity of isolated ergosterol peroxide against. The antiproliferative activity of isolated ergosterol peroxide against LS180 humancolon cancer cells was cells determined
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