Abstract

A simple method for the identification of sugars (and reducing substances) in melituric urine by paper electrophoresis at high potential gradients is described. Ten μl of the urine mixture is treated and the color is developed with a silver solution. Glycosuric urine showed three distinctly separated bands of glucose, uric acid and urea-creatinine. Glucose fructose, ribose, rhamnose, lactose, maltose, sucrose, glucuronic acid and glucosamine were easily identified by this technique. Sorbose and xylose were difficult to differentiate from glucose since they gave bands close to the glucose band. Overlapping occurred between the bands of galactose and fructose and of ribose and mannose. The method is recommended to supplement conventional methods of examining melituric urine.

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