Abstract
Despite the high efficiency of self-regeneration in liver, liver stem cell organoid culture is way behind other cell types such as intestinal organoid (Enteroid). The contributing factor is the lack of effi¬cient technique to isolate liver cells and to culture liver stem cell organoid. Here we reported a new, eco¬nomical, and simple method to generate 3D hepatocyte organoid (Hepatoid) and cholangiocyte organoid (Cholangoid). We have isolated liver cells using ice-cold 3mM EDTA PBS medium. The isolated cells were cultured on 50% Conditional Medium. After 1 or 4 days post isolation and culture, Cholangoid and Hepatoid were readily to be detected under light microscope and they continued to grow well after several passages. This method could be used to isolate and culture human liver organoids. This feasible protocol will greatly facilitate more research on liver function and diseases using liver cell organoids. Keywords: Organoid; Stem cell; 3D culture; Liver
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Clinical Gastroenterology and Hepatology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
