Abstract
A new kinetic method has been developed for the determination of nitrite in fruit juice samples. The method is based on the catalytic effect of nitrite with the oxidation of Nile Blue A (NBA) by KBrO(3) in the sulfuric acid medium. The optimum conditions obtained are 1.2mM sulfuric acid, 0.034mM of NBA, 2.8 × 10(-3)M KBrO(3), reaction temperature of 20°C, and reaction time of 100s at 595.5nm. Under the optimized conditions, the method allowed the quantification of nitrite in a range of 0.2-800μg/mL with a detection limit of 0.02μg/mL. The method was applied to the determination of nitrite in 15 brands of fruit juice samples.
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