Abstract
The effects of magnesium, calcium, and barium ions on the catalytic activity of alkaline phosphatase from the small intestine of the Greenland seal in the reaction of p-nitrophenyl phosphate hydrolysis in the presence of a number of complexones (ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, 1,2-cyclohexanediaminetetraacetic acid, and nitrilotrimethylenephosphonic acid) were studied. As a result, a new approach to the enzymatic determination of the above metals was developed. It consists in the use of the liberative effects of these metals on alkaline phosphatase preinhibited by nitrilotrimethylenephosphonic acid. Sensitive procedures for the enzymatic determination of magnesium, calcium, and barium ions were developed (c min = 14 ng/mL, 24 ng/mL, and 0.8 µg/mL, respectively).
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