Abstract
The first example of simultaneous triple carbonylmetalloimmunoassay (CMIA), a non-isotopic immunological method, is presented herein. This method, originally developed for mono-assays, uses various transition metal carbonyl complexes as labels and sensitive quantitation of these tracers by Fourier transform infrared (FT-IR) spectroscopy, thanks to the characteristic νCO bands appearing in the 1800–2200 cm−1 region. We show here that this method offers an advance in the challenging field of simultaneous multi-immunoassay, with the example of the assay of three antiepileptic drugs (carbamazepine, phenobarbital and diphenylhydantoin).
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