Abstract
Protein separation has been the most challenging issue of biochemists since the Antoine François, comte de Fourcroy's first attempts to isolate some proteins from plants by precipitation in 1789. Protein purification methods then developed simultaneously with the deep understanding of the properties and the functions of proteins. One of the most important milestones in the history of protein purification is the development of ‘chromatography’ in 1903 by Tswett. Since then, various forms of chromatography have become a powerful and essential tool for the separation of biomolecules in combination with other techniques such as precipitation and ultracentrifugation or even by only itself.
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