Abstract
The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry. The results clearly demonstrate that water-soluble C60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells. C60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells.
Highlights
The water-soluble inorganic bi-valent platinum derivative, cisplatin (cis-[Pt(II)(NH3)2Cl2], Cis), is currently one of the most effective therapeutic agents used against cancer deceases, in particular, ovarian cancer, bladder cancer, esophagus cancer, lung cancer, and cancer of head and neck [1]
The results clearly demonstrate that water-soluble C60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells
In order to testify this hypothesis in the present study we evaluated and compared in vitro cytotoxic action of C60 fullerene, Сіs and their complex on lymphocytes from healthy persons, as well as their genotoxic effects towards resting lymphocytes and lymphocytes after blast transformation
Summary
The water-soluble inorganic bi-valent platinum derivative, cisplatin (cis-[Pt(II)(NH3)2Cl2], Cis), is currently one of the most effective therapeutic agents used against cancer deceases, in particular, ovarian cancer, bladder cancer, esophagus cancer, lung cancer, and cancer of head and neck [1]. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. C60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells.
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