Abstract
Nonvaccine serotypes are increasingly responsible for pneumococcal empyema. We developed a one-step polymerase chain reaction for identifying all serotypes covered by the 13-valent conjugate vaccine. Over 1 year, we identified serotypes 19A ( n = 6), 1 ( n = 2), 3 ( n = 1) and 7F/A ( n = 2), both in culture-positive and culture-negative pleural fluid from 11 children with empyema.
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